megakaryoblastic cell line meg 01  (ATCC)

Journal: Malaria Journal

Article Title: Interactions between Plasmodium falciparum –infected red blood cells and their extracellular vesicles with megakaryocytes: implications for platelet-like particle formation

doi: 10.1186/s12936-025-05743-6

Figure Lengend Snippet: Binding of RBCs and RBC-EVs to MEG-01 cells after 5 h and overnight incubation. a Confocal images showing MEG-01 cells (cytoplasm stained with CTDR; magenta) and RBCs (membrane stained with PKH26; yellow). Scale bar: 10 µm (100 ×). b Quantification of binding between CTDR-labeled MEG-01 cells and PKH26-labeled RBCs or RBC-EVs using ImageJ. Binding events were counted in 10 randomly selected fields or normalized per 1,000 adherent MEG-01 cells at 5 h and overnight. c Z-stack confocal images showing binding interactions between MEG-01 cells and either RBCs or RBC-EVs after overnight co-culture. d Flow cytometry gating of double-positive nRBCs, pRBCs, nRBC-EVs, and pRBC-EVs bound to adherent or floating MEG-01 cells. e , f Bar graphs showing the percentage of CTDR-positive adherent and floating MEG-01 cells (+ FBS/+ PMA condition) after co-incubation with PKH26-labeled nRBCs/pRBCs ( e ) or nRBC-EVs/pRBC-EVs ( f ) for 5 h and overnight; n = 3. Statistical significance: ****p < 0.001 between conditions; ###p < 0.005, ####p < 0.001 between time points within the same condition; ns not significant

Article Snippet: The human megakaryoblastic cell line MEG-01 (Merck Life Science, Australia) was maintained in RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 10% heat-inactivated FBS.

Techniques: Binding Assay, Incubation, Staining, Membrane, Labeling, Co-Culture Assay, Flow Cytometry

Journal: Malaria Journal

Article Title: Interactions between Plasmodium falciparum –infected red blood cells and their extracellular vesicles with megakaryocytes: implications for platelet-like particle formation

doi: 10.1186/s12936-025-05743-6

Figure Lengend Snippet: Expression of surface markers on MEG-01 cells under different culture conditions and during co-culture with RBCs. Flow cytometry was used to assess surface marker expression on MEG-01 cells cultured under various conditions for 5 days. a – g Bar graphs showing the expression of CD41a, CD42b, CD61, CD41a⁺CD42b⁺, CD41a⁺CD61⁺ double-positive cells, Notch3, and Annexin V in MEG-01 cells cultured with or without FBS and PMA; n = 4. h – n Expression of the same markers in MEG-01 cells co-cultured with nRBCs or pRBCs under − FBS/+ PMA and + FBS/+ PMA conditions. All marker levels are expressed as the percentage of positive MEG-01 cells; n = 4. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001 between conditions; #p < 0.05, ####p < 0.001 between condition within the same treatments; ns not significant

Article Snippet: The human megakaryoblastic cell line MEG-01 (Merck Life Science, Australia) was maintained in RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 10% heat-inactivated FBS.

Techniques: Expressing, Co-Culture Assay, Flow Cytometry, Marker, Cell Culture

Journal: Malaria Journal

Article Title: Interactions between Plasmodium falciparum –infected red blood cells and their extracellular vesicles with megakaryocytes: implications for platelet-like particle formation

doi: 10.1186/s12936-025-05743-6

Figure Lengend Snippet: Expression of surface markers on PLPs derived from MEG-01 cells under different culture conditions and during co-culture with RBCs. Flow cytometry was used to assess the expression of surface markers on PLPs collected from MEG-01 cultures for 5 days. a – g Bar graphs showing the expression of CD41a, CD42b, CD61, CD41a⁺CD42b⁺, CD41a⁺CD61⁺ double-positive cells, Notch3, and Annexin V in PLPs generated with or without FBS and PMA stimulation; n = 4. h – n Expression of the same markers in PLPs derived from MEG-01 cells co-cultured with nRBCs or pRBCs under − FBS/+ PMA and + FBS/+ PMA conditions; n = 4. All values represent the percentage of marker-positive PLPs. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001 between conditions; ##p < 0.01, ###p < 0.005, ####p < 0.001 between condition within the same treatments; ns not significant

Article Snippet: The human megakaryoblastic cell line MEG-01 (Merck Life Science, Australia) was maintained in RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 10% heat-inactivated FBS.

Techniques: Expressing, Derivative Assay, Co-Culture Assay, Flow Cytometry, Generated, Cell Culture, Marker

Journal: Malaria Journal

Article Title: Interactions between Plasmodium falciparum –infected red blood cells and their extracellular vesicles with megakaryocytes: implications for platelet-like particle formation

doi: 10.1186/s12936-025-05743-6

Figure Lengend Snippet: Production and functional activity of PLPs derived from MEG-01 cells under different culture conditions and during co-culture with RBCs or RBC-EVs. PLPs were collected from culture supernatants by centrifugation at 500 × g for 10 min and counted using a Malassez chamber. a Bar graph showing the number of PLPs per 500,000 MEG-01 cells on days 3 and 5 under different culture conditions; n = 3. b PLP counts from MEG-01 cells co-cultured with nRBCs or pRBCs with or without FBS and PMA; n = 3. c Similar analysis for co-cultures with nRBC-EVs or pRBC-EVs; n = 3. d Clotting activity of PLPs expressed as percentage of clotting time relative to platelet-poor plasma (PPP; negative control) and platelet-rich plasma (PRP; positive control) on days 3 and 5; n = 6. e Clotting time of PLPs produced with nRBCs or pRBCs was compared between − FBS/+ PMA and + FBS/+ PMA conditions; n = 6. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001 between conditions; #p < 0.05, ###p < 0.005, ####p < 0.001 within the same condition; ns not significant

Article Snippet: The human megakaryoblastic cell line MEG-01 (Merck Life Science, Australia) was maintained in RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 10% heat-inactivated FBS.

Techniques: Functional Assay, Activity Assay, Derivative Assay, Co-Culture Assay, Centrifugation, Cell Culture, Coagulation, Clinical Proteomics, Negative Control, Positive Control, Produced

Journal: Malaria Journal

Article Title: Interactions between Plasmodium falciparum –infected red blood cells and their extracellular vesicles with megakaryocytes: implications for platelet-like particle formation

doi: 10.1186/s12936-025-05743-6

Figure Lengend Snippet: Gene expression in MEG-01 cells under different culture conditions and during co-culture with RBCs. Relative gene expression in MEG-01 cells was analyzed on day 5 under various conditions, with or without FBS and PMA stimulation, and during co-culture with RBCs. a–j Bar graphs showing expression of key genes in MEG-01 cells cultured under four conditions: DLL4 ( a ), which impairs terminal megakaryocytic differentiation; NOTCH3 ( b ), a proliferation regulator; PDGFRB ( c ), associated with platelet function; BAX ( d ) and BCL2 ( e ), apoptosis regulators; CASPASE3 ( f ) and CASPASE9 ( g ), pro-apoptotic markers; ATG7 ( h ), MTOR ( i ), and NOX1 ( j ), related to autophagy and oxidative stress. k–t Expression of the same genes in MEG-01 cells co-cultured with nRBCs or pRBCs under − FBS/+ PMA and + FBS/+ PMA conditions; n = 3. Gene expression was quantified by RT-PCR and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are expressed as relative fold changes, with + FBS/− PMA set to 1. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001 between conditions

Article Snippet: The human megakaryoblastic cell line MEG-01 (Merck Life Science, Australia) was maintained in RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 10% heat-inactivated FBS.

Techniques: Gene Expression, Co-Culture Assay, Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction

Journal: Malaria Journal

Article Title: Interactions between Plasmodium falciparum –infected red blood cells and their extracellular vesicles with megakaryocytes: implications for platelet-like particle formation

doi: 10.1186/s12936-025-05743-6

Figure Lengend Snippet: Cytokine profiling in MEG-01 cultures under different conditions and during co-culture with RBCs or RBC-EVs. Cytokine concentrations were measured in MEG-01 culture supernatants collected on day 3 under various conditions. a–d Bar graphs showing concentrations of human IL-8 ( a ), MIP-1α ( b ), RANTES ( c ), and MCP-1 ( d ) under different culture conditions. e–h Cytokine levels in cultures co-incubated with nRBCs or pRBCs in the presence or absence of FBS and PMA. i–l Cytokine levels in cultures co-incubated with nRBC-EVs or pRBC-EVs under the same conditions; n = 6. All values represent cytokine concentrations (pg/ml). Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001 between conditions

Article Snippet: The human megakaryoblastic cell line MEG-01 (Merck Life Science, Australia) was maintained in RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 10% heat-inactivated FBS.

Techniques: Co-Culture Assay, Incubation